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dulbecco s modified eagle s medium dmem basic  (MedChemExpress)


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    Structured Review

    MedChemExpress dulbecco s modified eagle s medium dmem basic
    Dulbecco S Modified Eagle S Medium Dmem Basic, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 230 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/basic+medium+dmem/DMEM/pmc12320169-33-7-16
    Average 97 stars, based on 230 article reviews
    dulbecco s modified eagle s medium dmem basic - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Virus:

    Article Title: Pyruvate metabolism enzyme DLAT promotes tumorigenesis by suppressing leucine catabolism.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Rosetta WEIDI Cat# EC1010 TOP10 WEIDI Cat# DL1010 Stbl3 WEIDI Cat# DL1080 Lenti-virus This paper N/A AAV2/9-CBG-DIO-miR30 shNC Heyuan HYKY-240813020 AAV2/9-CBG-DIO-miR30 shRNA (DLAT) Heyuan HYKY-240813020 Biological samples Human Liver Tissue Sample Shanghai Jiao Tong University Affiliated Sixth People’s Hospital N/A Chemicals, peptides, and recombinant proteins Recombinant Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1) Abmart Cat# RKRD68955S DEN Sigma-Aldrich Cat# N0258 b-Methylcrotonyl coenzyme A lithium Sigma-Aldrich Cat# M3013 b-hydroxy-b-mathylglutaryl-CoA Sigma-Aldrich Cat# H6132 L-Valine MedChemExpress Cat# HY-N0717 L-Leucine MedChemExpress Cat# HY-N0486 L-Aspartic acid MedChemExpress Cat# HY-N0666 DMEM basic (Amino acid Free) Yuchun Cat# YC-2082 L-Leucine (13C6; 99%) Cambridge Isotope Laboratories Cat# CLM-2262-H-PK Sodium Pyruvate (13C3; 99%) Cambridge Isotope Laboratories Cat# CLM-2440-PK L-Glutamine (13C5, 99%) Cambridge Isotope Laboratories Cat# CLM-1822-H-MG-PK Acetyl CoA (98%) Yuanye Cat# S23156 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat# L3000015 ECL detection reagent Mine Cat# WB0500 Trypsin Thermo Fisher Scientific Cat# 25200 Fetal Bovine Serum Thermo Fisher Scientific Cat# 16000-044 Protein A/G Magnetic Beads Thermo Fisher Scientific Cat# 88803 Anti-Flag Magnetic Beads Thermo Fisher Scientific Cat# A36797 Fetal Bovine Serum (Dialyzed) Thermo Fisher Scientific Cat# 30067334 PageRuler protein marker Thermo Fisher Scientific Cat# #26616/26619 Matrigel Corning Cat# 356234 DAPI Beyotime Cat# C1002 Deacetylase Inhibitor Cocktail, 100X Beyotime Cat# P1112 Protease Inhibitor Cocktail (100X in DMSO, EDTA Free) Mine Cat# MPI010 Critical commercial assays BCA assay kit Thermo Fisher Scientific Cat# 23225 BeyoClick EdU Cell Proliferation Kit with Alexa Fluor 555 Beyotime Cat# S0511S CCK8 Assay Kit Agilent Cat# 103792-100 Amino Acid Uptake Assay Kit DOJINDO Cat# UP04 Acetlytransferase Activity Kit Enzo Cat# ELS-ADI-907-026 Deposited data Raw and analyzed data Lu et al., 202262 GEO: GSE149614 Raw and analyzed data Kim et al., 201463 GEO: GSE39791 Raw and analyzed data Woo et al., 201764 GEO: GSE87630 Raw and analyzed data Roessler et al., 201065 GEO: GSE14520 Raw and analyzed data Wang et al., 201766 GEO: GSE84402 (Continued on next page) Cell Metabolism 37, 1–19.e1–e9, June 3, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Raw and analyzed data Whitby et al.19 https://doi.org/10.3390/cancers15215182 Acetylated proteomics data related to Figure 5 This paper Mendeley Data (https://doi.org/10.17632/ dsv57bhf65.1) Metabolomics raw data related to Figure 3A This paper Mendeley Data (https://doi.org/10.17632/ 69yx4r728s.1) RNA-seq of Homo sapiens strain: HepG2 cell line (sgDLAT vs sgNC) Raw sequence reads, related to Figure 4.

    shRNA:

    Article Title: Pyruvate metabolism enzyme DLAT promotes tumorigenesis by suppressing leucine catabolism.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Rosetta WEIDI Cat# EC1010 TOP10 WEIDI Cat# DL1010 Stbl3 WEIDI Cat# DL1080 Lenti-virus This paper N/A AAV2/9-CBG-DIO-miR30 shNC Heyuan HYKY-240813020 AAV2/9-CBG-DIO-miR30 shRNA (DLAT) Heyuan HYKY-240813020 Biological samples Human Liver Tissue Sample Shanghai Jiao Tong University Affiliated Sixth People’s Hospital N/A Chemicals, peptides, and recombinant proteins Recombinant Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1) Abmart Cat# RKRD68955S DEN Sigma-Aldrich Cat# N0258 b-Methylcrotonyl coenzyme A lithium Sigma-Aldrich Cat# M3013 b-hydroxy-b-mathylglutaryl-CoA Sigma-Aldrich Cat# H6132 L-Valine MedChemExpress Cat# HY-N0717 L-Leucine MedChemExpress Cat# HY-N0486 L-Aspartic acid MedChemExpress Cat# HY-N0666 DMEM basic (Amino acid Free) Yuchun Cat# YC-2082 L-Leucine (13C6; 99%) Cambridge Isotope Laboratories Cat# CLM-2262-H-PK Sodium Pyruvate (13C3; 99%) Cambridge Isotope Laboratories Cat# CLM-2440-PK L-Glutamine (13C5, 99%) Cambridge Isotope Laboratories Cat# CLM-1822-H-MG-PK Acetyl CoA (98%) Yuanye Cat# S23156 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat# L3000015 ECL detection reagent Mine Cat# WB0500 Trypsin Thermo Fisher Scientific Cat# 25200 Fetal Bovine Serum Thermo Fisher Scientific Cat# 16000-044 Protein A/G Magnetic Beads Thermo Fisher Scientific Cat# 88803 Anti-Flag Magnetic Beads Thermo Fisher Scientific Cat# A36797 Fetal Bovine Serum (Dialyzed) Thermo Fisher Scientific Cat# 30067334 PageRuler protein marker Thermo Fisher Scientific Cat# #26616/26619 Matrigel Corning Cat# 356234 DAPI Beyotime Cat# C1002 Deacetylase Inhibitor Cocktail, 100X Beyotime Cat# P1112 Protease Inhibitor Cocktail (100X in DMSO, EDTA Free) Mine Cat# MPI010 Critical commercial assays BCA assay kit Thermo Fisher Scientific Cat# 23225 BeyoClick EdU Cell Proliferation Kit with Alexa Fluor 555 Beyotime Cat# S0511S CCK8 Assay Kit Agilent Cat# 103792-100 Amino Acid Uptake Assay Kit DOJINDO Cat# UP04 Acetlytransferase Activity Kit Enzo Cat# ELS-ADI-907-026 Deposited data Raw and analyzed data Lu et al., 202262 GEO: GSE149614 Raw and analyzed data Kim et al., 201463 GEO: GSE39791 Raw and analyzed data Woo et al., 201764 GEO: GSE87630 Raw and analyzed data Roessler et al., 201065 GEO: GSE14520 Raw and analyzed data Wang et al., 201766 GEO: GSE84402 (Continued on next page) Cell Metabolism 37, 1–19.e1–e9, June 3, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Raw and analyzed data Whitby et al.19 https://doi.org/10.3390/cancers15215182 Acetylated proteomics data related to Figure 5 This paper Mendeley Data (https://doi.org/10.17632/ dsv57bhf65.1) Metabolomics raw data related to Figure 3A This paper Mendeley Data (https://doi.org/10.17632/ 69yx4r728s.1) RNA-seq of Homo sapiens strain: HepG2 cell line (sgDLAT vs sgNC) Raw sequence reads, related to Figure 4.

    Recombinant:

    Article Title: Pyruvate metabolism enzyme DLAT promotes tumorigenesis by suppressing leucine catabolism.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Rosetta WEIDI Cat# EC1010 TOP10 WEIDI Cat# DL1010 Stbl3 WEIDI Cat# DL1080 Lenti-virus This paper N/A AAV2/9-CBG-DIO-miR30 shNC Heyuan HYKY-240813020 AAV2/9-CBG-DIO-miR30 shRNA (DLAT) Heyuan HYKY-240813020 Biological samples Human Liver Tissue Sample Shanghai Jiao Tong University Affiliated Sixth People’s Hospital N/A Chemicals, peptides, and recombinant proteins Recombinant Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1) Abmart Cat# RKRD68955S DEN Sigma-Aldrich Cat# N0258 b-Methylcrotonyl coenzyme A lithium Sigma-Aldrich Cat# M3013 b-hydroxy-b-mathylglutaryl-CoA Sigma-Aldrich Cat# H6132 L-Valine MedChemExpress Cat# HY-N0717 L-Leucine MedChemExpress Cat# HY-N0486 L-Aspartic acid MedChemExpress Cat# HY-N0666 DMEM basic (Amino acid Free) Yuchun Cat# YC-2082 L-Leucine (13C6; 99%) Cambridge Isotope Laboratories Cat# CLM-2262-H-PK Sodium Pyruvate (13C3; 99%) Cambridge Isotope Laboratories Cat# CLM-2440-PK L-Glutamine (13C5, 99%) Cambridge Isotope Laboratories Cat# CLM-1822-H-MG-PK Acetyl CoA (98%) Yuanye Cat# S23156 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat# L3000015 ECL detection reagent Mine Cat# WB0500 Trypsin Thermo Fisher Scientific Cat# 25200 Fetal Bovine Serum Thermo Fisher Scientific Cat# 16000-044 Protein A/G Magnetic Beads Thermo Fisher Scientific Cat# 88803 Anti-Flag Magnetic Beads Thermo Fisher Scientific Cat# A36797 Fetal Bovine Serum (Dialyzed) Thermo Fisher Scientific Cat# 30067334 PageRuler protein marker Thermo Fisher Scientific Cat# #26616/26619 Matrigel Corning Cat# 356234 DAPI Beyotime Cat# C1002 Deacetylase Inhibitor Cocktail, 100X Beyotime Cat# P1112 Protease Inhibitor Cocktail (100X in DMSO, EDTA Free) Mine Cat# MPI010 Critical commercial assays BCA assay kit Thermo Fisher Scientific Cat# 23225 BeyoClick EdU Cell Proliferation Kit with Alexa Fluor 555 Beyotime Cat# S0511S CCK8 Assay Kit Agilent Cat# 103792-100 Amino Acid Uptake Assay Kit DOJINDO Cat# UP04 Acetlytransferase Activity Kit Enzo Cat# ELS-ADI-907-026 Deposited data Raw and analyzed data Lu et al., 202262 GEO: GSE149614 Raw and analyzed data Kim et al., 201463 GEO: GSE39791 Raw and analyzed data Woo et al., 201764 GEO: GSE87630 Raw and analyzed data Roessler et al., 201065 GEO: GSE14520 Raw and analyzed data Wang et al., 201766 GEO: GSE84402 (Continued on next page) Cell Metabolism 37, 1–19.e1–e9, June 3, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Raw and analyzed data Whitby et al.19 https://doi.org/10.3390/cancers15215182 Acetylated proteomics data related to Figure 5 This paper Mendeley Data (https://doi.org/10.17632/ dsv57bhf65.1) Metabolomics raw data related to Figure 3A This paper Mendeley Data (https://doi.org/10.17632/ 69yx4r728s.1) RNA-seq of Homo sapiens strain: HepG2 cell line (sgDLAT vs sgNC) Raw sequence reads, related to Figure 4.

    Transfection:

    Article Title: Pyruvate metabolism enzyme DLAT promotes tumorigenesis by suppressing leucine catabolism.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Rosetta WEIDI Cat# EC1010 TOP10 WEIDI Cat# DL1010 Stbl3 WEIDI Cat# DL1080 Lenti-virus This paper N/A AAV2/9-CBG-DIO-miR30 shNC Heyuan HYKY-240813020 AAV2/9-CBG-DIO-miR30 shRNA (DLAT) Heyuan HYKY-240813020 Biological samples Human Liver Tissue Sample Shanghai Jiao Tong University Affiliated Sixth People’s Hospital N/A Chemicals, peptides, and recombinant proteins Recombinant Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1) Abmart Cat# RKRD68955S DEN Sigma-Aldrich Cat# N0258 b-Methylcrotonyl coenzyme A lithium Sigma-Aldrich Cat# M3013 b-hydroxy-b-mathylglutaryl-CoA Sigma-Aldrich Cat# H6132 L-Valine MedChemExpress Cat# HY-N0717 L-Leucine MedChemExpress Cat# HY-N0486 L-Aspartic acid MedChemExpress Cat# HY-N0666 DMEM basic (Amino acid Free) Yuchun Cat# YC-2082 L-Leucine (13C6; 99%) Cambridge Isotope Laboratories Cat# CLM-2262-H-PK Sodium Pyruvate (13C3; 99%) Cambridge Isotope Laboratories Cat# CLM-2440-PK L-Glutamine (13C5, 99%) Cambridge Isotope Laboratories Cat# CLM-1822-H-MG-PK Acetyl CoA (98%) Yuanye Cat# S23156 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat# L3000015 ECL detection reagent Mine Cat# WB0500 Trypsin Thermo Fisher Scientific Cat# 25200 Fetal Bovine Serum Thermo Fisher Scientific Cat# 16000-044 Protein A/G Magnetic Beads Thermo Fisher Scientific Cat# 88803 Anti-Flag Magnetic Beads Thermo Fisher Scientific Cat# A36797 Fetal Bovine Serum (Dialyzed) Thermo Fisher Scientific Cat# 30067334 PageRuler protein marker Thermo Fisher Scientific Cat# #26616/26619 Matrigel Corning Cat# 356234 DAPI Beyotime Cat# C1002 Deacetylase Inhibitor Cocktail, 100X Beyotime Cat# P1112 Protease Inhibitor Cocktail (100X in DMSO, EDTA Free) Mine Cat# MPI010 Critical commercial assays BCA assay kit Thermo Fisher Scientific Cat# 23225 BeyoClick EdU Cell Proliferation Kit with Alexa Fluor 555 Beyotime Cat# S0511S CCK8 Assay Kit Agilent Cat# 103792-100 Amino Acid Uptake Assay Kit DOJINDO Cat# UP04 Acetlytransferase Activity Kit Enzo Cat# ELS-ADI-907-026 Deposited data Raw and analyzed data Lu et al., 202262 GEO: GSE149614 Raw and analyzed data Kim et al., 201463 GEO: GSE39791 Raw and analyzed data Woo et al., 201764 GEO: GSE87630 Raw and analyzed data Roessler et al., 201065 GEO: GSE14520 Raw and analyzed data Wang et al., 201766 GEO: GSE84402 (Continued on next page) Cell Metabolism 37, 1–19.e1–e9, June 3, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Raw and analyzed data Whitby et al.19 https://doi.org/10.3390/cancers15215182 Acetylated proteomics data related to Figure 5 This paper Mendeley Data (https://doi.org/10.17632/ dsv57bhf65.1) Metabolomics raw data related to Figure 3A This paper Mendeley Data (https://doi.org/10.17632/ 69yx4r728s.1) RNA-seq of Homo sapiens strain: HepG2 cell line (sgDLAT vs sgNC) Raw sequence reads, related to Figure 4.

    Magnetic Beads:

    Article Title: Pyruvate metabolism enzyme DLAT promotes tumorigenesis by suppressing leucine catabolism.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Rosetta WEIDI Cat# EC1010 TOP10 WEIDI Cat# DL1010 Stbl3 WEIDI Cat# DL1080 Lenti-virus This paper N/A AAV2/9-CBG-DIO-miR30 shNC Heyuan HYKY-240813020 AAV2/9-CBG-DIO-miR30 shRNA (DLAT) Heyuan HYKY-240813020 Biological samples Human Liver Tissue Sample Shanghai Jiao Tong University Affiliated Sixth People’s Hospital N/A Chemicals, peptides, and recombinant proteins Recombinant Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1) Abmart Cat# RKRD68955S DEN Sigma-Aldrich Cat# N0258 b-Methylcrotonyl coenzyme A lithium Sigma-Aldrich Cat# M3013 b-hydroxy-b-mathylglutaryl-CoA Sigma-Aldrich Cat# H6132 L-Valine MedChemExpress Cat# HY-N0717 L-Leucine MedChemExpress Cat# HY-N0486 L-Aspartic acid MedChemExpress Cat# HY-N0666 DMEM basic (Amino acid Free) Yuchun Cat# YC-2082 L-Leucine (13C6; 99%) Cambridge Isotope Laboratories Cat# CLM-2262-H-PK Sodium Pyruvate (13C3; 99%) Cambridge Isotope Laboratories Cat# CLM-2440-PK L-Glutamine (13C5, 99%) Cambridge Isotope Laboratories Cat# CLM-1822-H-MG-PK Acetyl CoA (98%) Yuanye Cat# S23156 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat# L3000015 ECL detection reagent Mine Cat# WB0500 Trypsin Thermo Fisher Scientific Cat# 25200 Fetal Bovine Serum Thermo Fisher Scientific Cat# 16000-044 Protein A/G Magnetic Beads Thermo Fisher Scientific Cat# 88803 Anti-Flag Magnetic Beads Thermo Fisher Scientific Cat# A36797 Fetal Bovine Serum (Dialyzed) Thermo Fisher Scientific Cat# 30067334 PageRuler protein marker Thermo Fisher Scientific Cat# #26616/26619 Matrigel Corning Cat# 356234 DAPI Beyotime Cat# C1002 Deacetylase Inhibitor Cocktail, 100X Beyotime Cat# P1112 Protease Inhibitor Cocktail (100X in DMSO, EDTA Free) Mine Cat# MPI010 Critical commercial assays BCA assay kit Thermo Fisher Scientific Cat# 23225 BeyoClick EdU Cell Proliferation Kit with Alexa Fluor 555 Beyotime Cat# S0511S CCK8 Assay Kit Agilent Cat# 103792-100 Amino Acid Uptake Assay Kit DOJINDO Cat# UP04 Acetlytransferase Activity Kit Enzo Cat# ELS-ADI-907-026 Deposited data Raw and analyzed data Lu et al., 202262 GEO: GSE149614 Raw and analyzed data Kim et al., 201463 GEO: GSE39791 Raw and analyzed data Woo et al., 201764 GEO: GSE87630 Raw and analyzed data Roessler et al., 201065 GEO: GSE14520 Raw and analyzed data Wang et al., 201766 GEO: GSE84402 (Continued on next page) Cell Metabolism 37, 1–19.e1–e9, June 3, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Raw and analyzed data Whitby et al.19 https://doi.org/10.3390/cancers15215182 Acetylated proteomics data related to Figure 5 This paper Mendeley Data (https://doi.org/10.17632/ dsv57bhf65.1) Metabolomics raw data related to Figure 3A This paper Mendeley Data (https://doi.org/10.17632/ 69yx4r728s.1) RNA-seq of Homo sapiens strain: HepG2 cell line (sgDLAT vs sgNC) Raw sequence reads, related to Figure 4.

    Marker:

    Article Title: Pyruvate metabolism enzyme DLAT promotes tumorigenesis by suppressing leucine catabolism.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Rosetta WEIDI Cat# EC1010 TOP10 WEIDI Cat# DL1010 Stbl3 WEIDI Cat# DL1080 Lenti-virus This paper N/A AAV2/9-CBG-DIO-miR30 shNC Heyuan HYKY-240813020 AAV2/9-CBG-DIO-miR30 shRNA (DLAT) Heyuan HYKY-240813020 Biological samples Human Liver Tissue Sample Shanghai Jiao Tong University Affiliated Sixth People’s Hospital N/A Chemicals, peptides, and recombinant proteins Recombinant Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1) Abmart Cat# RKRD68955S DEN Sigma-Aldrich Cat# N0258 b-Methylcrotonyl coenzyme A lithium Sigma-Aldrich Cat# M3013 b-hydroxy-b-mathylglutaryl-CoA Sigma-Aldrich Cat# H6132 L-Valine MedChemExpress Cat# HY-N0717 L-Leucine MedChemExpress Cat# HY-N0486 L-Aspartic acid MedChemExpress Cat# HY-N0666 DMEM basic (Amino acid Free) Yuchun Cat# YC-2082 L-Leucine (13C6; 99%) Cambridge Isotope Laboratories Cat# CLM-2262-H-PK Sodium Pyruvate (13C3; 99%) Cambridge Isotope Laboratories Cat# CLM-2440-PK L-Glutamine (13C5, 99%) Cambridge Isotope Laboratories Cat# CLM-1822-H-MG-PK Acetyl CoA (98%) Yuanye Cat# S23156 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat# L3000015 ECL detection reagent Mine Cat# WB0500 Trypsin Thermo Fisher Scientific Cat# 25200 Fetal Bovine Serum Thermo Fisher Scientific Cat# 16000-044 Protein A/G Magnetic Beads Thermo Fisher Scientific Cat# 88803 Anti-Flag Magnetic Beads Thermo Fisher Scientific Cat# A36797 Fetal Bovine Serum (Dialyzed) Thermo Fisher Scientific Cat# 30067334 PageRuler protein marker Thermo Fisher Scientific Cat# #26616/26619 Matrigel Corning Cat# 356234 DAPI Beyotime Cat# C1002 Deacetylase Inhibitor Cocktail, 100X Beyotime Cat# P1112 Protease Inhibitor Cocktail (100X in DMSO, EDTA Free) Mine Cat# MPI010 Critical commercial assays BCA assay kit Thermo Fisher Scientific Cat# 23225 BeyoClick EdU Cell Proliferation Kit with Alexa Fluor 555 Beyotime Cat# S0511S CCK8 Assay Kit Agilent Cat# 103792-100 Amino Acid Uptake Assay Kit DOJINDO Cat# UP04 Acetlytransferase Activity Kit Enzo Cat# ELS-ADI-907-026 Deposited data Raw and analyzed data Lu et al., 202262 GEO: GSE149614 Raw and analyzed data Kim et al., 201463 GEO: GSE39791 Raw and analyzed data Woo et al., 201764 GEO: GSE87630 Raw and analyzed data Roessler et al., 201065 GEO: GSE14520 Raw and analyzed data Wang et al., 201766 GEO: GSE84402 (Continued on next page) Cell Metabolism 37, 1–19.e1–e9, June 3, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Raw and analyzed data Whitby et al.19 https://doi.org/10.3390/cancers15215182 Acetylated proteomics data related to Figure 5 This paper Mendeley Data (https://doi.org/10.17632/ dsv57bhf65.1) Metabolomics raw data related to Figure 3A This paper Mendeley Data (https://doi.org/10.17632/ 69yx4r728s.1) RNA-seq of Homo sapiens strain: HepG2 cell line (sgDLAT vs sgNC) Raw sequence reads, related to Figure 4.

    Histone Deacetylase Assay:

    Article Title: Pyruvate metabolism enzyme DLAT promotes tumorigenesis by suppressing leucine catabolism.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Rosetta WEIDI Cat# EC1010 TOP10 WEIDI Cat# DL1010 Stbl3 WEIDI Cat# DL1080 Lenti-virus This paper N/A AAV2/9-CBG-DIO-miR30 shNC Heyuan HYKY-240813020 AAV2/9-CBG-DIO-miR30 shRNA (DLAT) Heyuan HYKY-240813020 Biological samples Human Liver Tissue Sample Shanghai Jiao Tong University Affiliated Sixth People’s Hospital N/A Chemicals, peptides, and recombinant proteins Recombinant Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1) Abmart Cat# RKRD68955S DEN Sigma-Aldrich Cat# N0258 b-Methylcrotonyl coenzyme A lithium Sigma-Aldrich Cat# M3013 b-hydroxy-b-mathylglutaryl-CoA Sigma-Aldrich Cat# H6132 L-Valine MedChemExpress Cat# HY-N0717 L-Leucine MedChemExpress Cat# HY-N0486 L-Aspartic acid MedChemExpress Cat# HY-N0666 DMEM basic (Amino acid Free) Yuchun Cat# YC-2082 L-Leucine (13C6; 99%) Cambridge Isotope Laboratories Cat# CLM-2262-H-PK Sodium Pyruvate (13C3; 99%) Cambridge Isotope Laboratories Cat# CLM-2440-PK L-Glutamine (13C5, 99%) Cambridge Isotope Laboratories Cat# CLM-1822-H-MG-PK Acetyl CoA (98%) Yuanye Cat# S23156 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat# L3000015 ECL detection reagent Mine Cat# WB0500 Trypsin Thermo Fisher Scientific Cat# 25200 Fetal Bovine Serum Thermo Fisher Scientific Cat# 16000-044 Protein A/G Magnetic Beads Thermo Fisher Scientific Cat# 88803 Anti-Flag Magnetic Beads Thermo Fisher Scientific Cat# A36797 Fetal Bovine Serum (Dialyzed) Thermo Fisher Scientific Cat# 30067334 PageRuler protein marker Thermo Fisher Scientific Cat# #26616/26619 Matrigel Corning Cat# 356234 DAPI Beyotime Cat# C1002 Deacetylase Inhibitor Cocktail, 100X Beyotime Cat# P1112 Protease Inhibitor Cocktail (100X in DMSO, EDTA Free) Mine Cat# MPI010 Critical commercial assays BCA assay kit Thermo Fisher Scientific Cat# 23225 BeyoClick EdU Cell Proliferation Kit with Alexa Fluor 555 Beyotime Cat# S0511S CCK8 Assay Kit Agilent Cat# 103792-100 Amino Acid Uptake Assay Kit DOJINDO Cat# UP04 Acetlytransferase Activity Kit Enzo Cat# ELS-ADI-907-026 Deposited data Raw and analyzed data Lu et al., 202262 GEO: GSE149614 Raw and analyzed data Kim et al., 201463 GEO: GSE39791 Raw and analyzed data Woo et al., 201764 GEO: GSE87630 Raw and analyzed data Roessler et al., 201065 GEO: GSE14520 Raw and analyzed data Wang et al., 201766 GEO: GSE84402 (Continued on next page) Cell Metabolism 37, 1–19.e1–e9, June 3, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Raw and analyzed data Whitby et al.19 https://doi.org/10.3390/cancers15215182 Acetylated proteomics data related to Figure 5 This paper Mendeley Data (https://doi.org/10.17632/ dsv57bhf65.1) Metabolomics raw data related to Figure 3A This paper Mendeley Data (https://doi.org/10.17632/ 69yx4r728s.1) RNA-seq of Homo sapiens strain: HepG2 cell line (sgDLAT vs sgNC) Raw sequence reads, related to Figure 4.

    Protease Inhibitor:

    Article Title: Pyruvate metabolism enzyme DLAT promotes tumorigenesis by suppressing leucine catabolism.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Rosetta WEIDI Cat# EC1010 TOP10 WEIDI Cat# DL1010 Stbl3 WEIDI Cat# DL1080 Lenti-virus This paper N/A AAV2/9-CBG-DIO-miR30 shNC Heyuan HYKY-240813020 AAV2/9-CBG-DIO-miR30 shRNA (DLAT) Heyuan HYKY-240813020 Biological samples Human Liver Tissue Sample Shanghai Jiao Tong University Affiliated Sixth People’s Hospital N/A Chemicals, peptides, and recombinant proteins Recombinant Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1) Abmart Cat# RKRD68955S DEN Sigma-Aldrich Cat# N0258 b-Methylcrotonyl coenzyme A lithium Sigma-Aldrich Cat# M3013 b-hydroxy-b-mathylglutaryl-CoA Sigma-Aldrich Cat# H6132 L-Valine MedChemExpress Cat# HY-N0717 L-Leucine MedChemExpress Cat# HY-N0486 L-Aspartic acid MedChemExpress Cat# HY-N0666 DMEM basic (Amino acid Free) Yuchun Cat# YC-2082 L-Leucine (13C6; 99%) Cambridge Isotope Laboratories Cat# CLM-2262-H-PK Sodium Pyruvate (13C3; 99%) Cambridge Isotope Laboratories Cat# CLM-2440-PK L-Glutamine (13C5, 99%) Cambridge Isotope Laboratories Cat# CLM-1822-H-MG-PK Acetyl CoA (98%) Yuanye Cat# S23156 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat# L3000015 ECL detection reagent Mine Cat# WB0500 Trypsin Thermo Fisher Scientific Cat# 25200 Fetal Bovine Serum Thermo Fisher Scientific Cat# 16000-044 Protein A/G Magnetic Beads Thermo Fisher Scientific Cat# 88803 Anti-Flag Magnetic Beads Thermo Fisher Scientific Cat# A36797 Fetal Bovine Serum (Dialyzed) Thermo Fisher Scientific Cat# 30067334 PageRuler protein marker Thermo Fisher Scientific Cat# #26616/26619 Matrigel Corning Cat# 356234 DAPI Beyotime Cat# C1002 Deacetylase Inhibitor Cocktail, 100X Beyotime Cat# P1112 Protease Inhibitor Cocktail (100X in DMSO, EDTA Free) Mine Cat# MPI010 Critical commercial assays BCA assay kit Thermo Fisher Scientific Cat# 23225 BeyoClick EdU Cell Proliferation Kit with Alexa Fluor 555 Beyotime Cat# S0511S CCK8 Assay Kit Agilent Cat# 103792-100 Amino Acid Uptake Assay Kit DOJINDO Cat# UP04 Acetlytransferase Activity Kit Enzo Cat# ELS-ADI-907-026 Deposited data Raw and analyzed data Lu et al., 202262 GEO: GSE149614 Raw and analyzed data Kim et al., 201463 GEO: GSE39791 Raw and analyzed data Woo et al., 201764 GEO: GSE87630 Raw and analyzed data Roessler et al., 201065 GEO: GSE14520 Raw and analyzed data Wang et al., 201766 GEO: GSE84402 (Continued on next page) Cell Metabolism 37, 1–19.e1–e9, June 3, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Raw and analyzed data Whitby et al.19 https://doi.org/10.3390/cancers15215182 Acetylated proteomics data related to Figure 5 This paper Mendeley Data (https://doi.org/10.17632/ dsv57bhf65.1) Metabolomics raw data related to Figure 3A This paper Mendeley Data (https://doi.org/10.17632/ 69yx4r728s.1) RNA-seq of Homo sapiens strain: HepG2 cell line (sgDLAT vs sgNC) Raw sequence reads, related to Figure 4.

    BIA-KA:

    Article Title: Pyruvate metabolism enzyme DLAT promotes tumorigenesis by suppressing leucine catabolism.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Rosetta WEIDI Cat# EC1010 TOP10 WEIDI Cat# DL1010 Stbl3 WEIDI Cat# DL1080 Lenti-virus This paper N/A AAV2/9-CBG-DIO-miR30 shNC Heyuan HYKY-240813020 AAV2/9-CBG-DIO-miR30 shRNA (DLAT) Heyuan HYKY-240813020 Biological samples Human Liver Tissue Sample Shanghai Jiao Tong University Affiliated Sixth People’s Hospital N/A Chemicals, peptides, and recombinant proteins Recombinant Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1) Abmart Cat# RKRD68955S DEN Sigma-Aldrich Cat# N0258 b-Methylcrotonyl coenzyme A lithium Sigma-Aldrich Cat# M3013 b-hydroxy-b-mathylglutaryl-CoA Sigma-Aldrich Cat# H6132 L-Valine MedChemExpress Cat# HY-N0717 L-Leucine MedChemExpress Cat# HY-N0486 L-Aspartic acid MedChemExpress Cat# HY-N0666 DMEM basic (Amino acid Free) Yuchun Cat# YC-2082 L-Leucine (13C6; 99%) Cambridge Isotope Laboratories Cat# CLM-2262-H-PK Sodium Pyruvate (13C3; 99%) Cambridge Isotope Laboratories Cat# CLM-2440-PK L-Glutamine (13C5, 99%) Cambridge Isotope Laboratories Cat# CLM-1822-H-MG-PK Acetyl CoA (98%) Yuanye Cat# S23156 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat# L3000015 ECL detection reagent Mine Cat# WB0500 Trypsin Thermo Fisher Scientific Cat# 25200 Fetal Bovine Serum Thermo Fisher Scientific Cat# 16000-044 Protein A/G Magnetic Beads Thermo Fisher Scientific Cat# 88803 Anti-Flag Magnetic Beads Thermo Fisher Scientific Cat# A36797 Fetal Bovine Serum (Dialyzed) Thermo Fisher Scientific Cat# 30067334 PageRuler protein marker Thermo Fisher Scientific Cat# #26616/26619 Matrigel Corning Cat# 356234 DAPI Beyotime Cat# C1002 Deacetylase Inhibitor Cocktail, 100X Beyotime Cat# P1112 Protease Inhibitor Cocktail (100X in DMSO, EDTA Free) Mine Cat# MPI010 Critical commercial assays BCA assay kit Thermo Fisher Scientific Cat# 23225 BeyoClick EdU Cell Proliferation Kit with Alexa Fluor 555 Beyotime Cat# S0511S CCK8 Assay Kit Agilent Cat# 103792-100 Amino Acid Uptake Assay Kit DOJINDO Cat# UP04 Acetlytransferase Activity Kit Enzo Cat# ELS-ADI-907-026 Deposited data Raw and analyzed data Lu et al., 202262 GEO: GSE149614 Raw and analyzed data Kim et al., 201463 GEO: GSE39791 Raw and analyzed data Woo et al., 201764 GEO: GSE87630 Raw and analyzed data Roessler et al., 201065 GEO: GSE14520 Raw and analyzed data Wang et al., 201766 GEO: GSE84402 (Continued on next page) Cell Metabolism 37, 1–19.e1–e9, June 3, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Raw and analyzed data Whitby et al.19 https://doi.org/10.3390/cancers15215182 Acetylated proteomics data related to Figure 5 This paper Mendeley Data (https://doi.org/10.17632/ dsv57bhf65.1) Metabolomics raw data related to Figure 3A This paper Mendeley Data (https://doi.org/10.17632/ 69yx4r728s.1) RNA-seq of Homo sapiens strain: HepG2 cell line (sgDLAT vs sgNC) Raw sequence reads, related to Figure 4.

    CCK-8 Assay:

    Article Title: Pyruvate metabolism enzyme DLAT promotes tumorigenesis by suppressing leucine catabolism.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Rosetta WEIDI Cat# EC1010 TOP10 WEIDI Cat# DL1010 Stbl3 WEIDI Cat# DL1080 Lenti-virus This paper N/A AAV2/9-CBG-DIO-miR30 shNC Heyuan HYKY-240813020 AAV2/9-CBG-DIO-miR30 shRNA (DLAT) Heyuan HYKY-240813020 Biological samples Human Liver Tissue Sample Shanghai Jiao Tong University Affiliated Sixth People’s Hospital N/A Chemicals, peptides, and recombinant proteins Recombinant Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1) Abmart Cat# RKRD68955S DEN Sigma-Aldrich Cat# N0258 b-Methylcrotonyl coenzyme A lithium Sigma-Aldrich Cat# M3013 b-hydroxy-b-mathylglutaryl-CoA Sigma-Aldrich Cat# H6132 L-Valine MedChemExpress Cat# HY-N0717 L-Leucine MedChemExpress Cat# HY-N0486 L-Aspartic acid MedChemExpress Cat# HY-N0666 DMEM basic (Amino acid Free) Yuchun Cat# YC-2082 L-Leucine (13C6; 99%) Cambridge Isotope Laboratories Cat# CLM-2262-H-PK Sodium Pyruvate (13C3; 99%) Cambridge Isotope Laboratories Cat# CLM-2440-PK L-Glutamine (13C5, 99%) Cambridge Isotope Laboratories Cat# CLM-1822-H-MG-PK Acetyl CoA (98%) Yuanye Cat# S23156 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat# L3000015 ECL detection reagent Mine Cat# WB0500 Trypsin Thermo Fisher Scientific Cat# 25200 Fetal Bovine Serum Thermo Fisher Scientific Cat# 16000-044 Protein A/G Magnetic Beads Thermo Fisher Scientific Cat# 88803 Anti-Flag Magnetic Beads Thermo Fisher Scientific Cat# A36797 Fetal Bovine Serum (Dialyzed) Thermo Fisher Scientific Cat# 30067334 PageRuler protein marker Thermo Fisher Scientific Cat# #26616/26619 Matrigel Corning Cat# 356234 DAPI Beyotime Cat# C1002 Deacetylase Inhibitor Cocktail, 100X Beyotime Cat# P1112 Protease Inhibitor Cocktail (100X in DMSO, EDTA Free) Mine Cat# MPI010 Critical commercial assays BCA assay kit Thermo Fisher Scientific Cat# 23225 BeyoClick EdU Cell Proliferation Kit with Alexa Fluor 555 Beyotime Cat# S0511S CCK8 Assay Kit Agilent Cat# 103792-100 Amino Acid Uptake Assay Kit DOJINDO Cat# UP04 Acetlytransferase Activity Kit Enzo Cat# ELS-ADI-907-026 Deposited data Raw and analyzed data Lu et al., 202262 GEO: GSE149614 Raw and analyzed data Kim et al., 201463 GEO: GSE39791 Raw and analyzed data Woo et al., 201764 GEO: GSE87630 Raw and analyzed data Roessler et al., 201065 GEO: GSE14520 Raw and analyzed data Wang et al., 201766 GEO: GSE84402 (Continued on next page) Cell Metabolism 37, 1–19.e1–e9, June 3, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Raw and analyzed data Whitby et al.19 https://doi.org/10.3390/cancers15215182 Acetylated proteomics data related to Figure 5 This paper Mendeley Data (https://doi.org/10.17632/ dsv57bhf65.1) Metabolomics raw data related to Figure 3A This paper Mendeley Data (https://doi.org/10.17632/ 69yx4r728s.1) RNA-seq of Homo sapiens strain: HepG2 cell line (sgDLAT vs sgNC) Raw sequence reads, related to Figure 4.

    Activity Assay:

    Article Title: Pyruvate metabolism enzyme DLAT promotes tumorigenesis by suppressing leucine catabolism.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Rosetta WEIDI Cat# EC1010 TOP10 WEIDI Cat# DL1010 Stbl3 WEIDI Cat# DL1080 Lenti-virus This paper N/A AAV2/9-CBG-DIO-miR30 shNC Heyuan HYKY-240813020 AAV2/9-CBG-DIO-miR30 shRNA (DLAT) Heyuan HYKY-240813020 Biological samples Human Liver Tissue Sample Shanghai Jiao Tong University Affiliated Sixth People’s Hospital N/A Chemicals, peptides, and recombinant proteins Recombinant Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1) Abmart Cat# RKRD68955S DEN Sigma-Aldrich Cat# N0258 b-Methylcrotonyl coenzyme A lithium Sigma-Aldrich Cat# M3013 b-hydroxy-b-mathylglutaryl-CoA Sigma-Aldrich Cat# H6132 L-Valine MedChemExpress Cat# HY-N0717 L-Leucine MedChemExpress Cat# HY-N0486 L-Aspartic acid MedChemExpress Cat# HY-N0666 DMEM basic (Amino acid Free) Yuchun Cat# YC-2082 L-Leucine (13C6; 99%) Cambridge Isotope Laboratories Cat# CLM-2262-H-PK Sodium Pyruvate (13C3; 99%) Cambridge Isotope Laboratories Cat# CLM-2440-PK L-Glutamine (13C5, 99%) Cambridge Isotope Laboratories Cat# CLM-1822-H-MG-PK Acetyl CoA (98%) Yuanye Cat# S23156 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat# L3000015 ECL detection reagent Mine Cat# WB0500 Trypsin Thermo Fisher Scientific Cat# 25200 Fetal Bovine Serum Thermo Fisher Scientific Cat# 16000-044 Protein A/G Magnetic Beads Thermo Fisher Scientific Cat# 88803 Anti-Flag Magnetic Beads Thermo Fisher Scientific Cat# A36797 Fetal Bovine Serum (Dialyzed) Thermo Fisher Scientific Cat# 30067334 PageRuler protein marker Thermo Fisher Scientific Cat# #26616/26619 Matrigel Corning Cat# 356234 DAPI Beyotime Cat# C1002 Deacetylase Inhibitor Cocktail, 100X Beyotime Cat# P1112 Protease Inhibitor Cocktail (100X in DMSO, EDTA Free) Mine Cat# MPI010 Critical commercial assays BCA assay kit Thermo Fisher Scientific Cat# 23225 BeyoClick EdU Cell Proliferation Kit with Alexa Fluor 555 Beyotime Cat# S0511S CCK8 Assay Kit Agilent Cat# 103792-100 Amino Acid Uptake Assay Kit DOJINDO Cat# UP04 Acetlytransferase Activity Kit Enzo Cat# ELS-ADI-907-026 Deposited data Raw and analyzed data Lu et al., 202262 GEO: GSE149614 Raw and analyzed data Kim et al., 201463 GEO: GSE39791 Raw and analyzed data Woo et al., 201764 GEO: GSE87630 Raw and analyzed data Roessler et al., 201065 GEO: GSE14520 Raw and analyzed data Wang et al., 201766 GEO: GSE84402 (Continued on next page) Cell Metabolism 37, 1–19.e1–e9, June 3, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Raw and analyzed data Whitby et al.19 https://doi.org/10.3390/cancers15215182 Acetylated proteomics data related to Figure 5 This paper Mendeley Data (https://doi.org/10.17632/ dsv57bhf65.1) Metabolomics raw data related to Figure 3A This paper Mendeley Data (https://doi.org/10.17632/ 69yx4r728s.1) RNA-seq of Homo sapiens strain: HepG2 cell line (sgDLAT vs sgNC) Raw sequence reads, related to Figure 4.

    Modification:

    Article Title: Hypoglycemic effects and chemical changes of mulberry fruits of different ripeness: In vitro and silico studies
    Article Snippet: DNS reagent, soluble starch and phosphate buffer solution (PBS) were purchased from Rhawn Chemical Reagent Co., LTD (Shanghai, China). .. Superior fetal bovine serum (FBS), penicillin-streptomycin and dulbecco's modified Eagle's medium (DMEM) basic were purchased from MedChemExpress LLC (Shanghai, China). .. Mass spectrometric grade methanol and acetonitrile were purchased from Fisher Chemical (Waltham, MA, USA).

    Cell Culture:

    Article Title: The gut microbial metabolite phenylacetylglutamine increases susceptibility to atrial fibrillation after myocardial infarction through ferroptosis and NLRP3 inflammasome.
    Article Snippet: Myocardial infarction (MI) is an important risk factor for the development of atrial fibrillation (AF), and the gut microbial metabolite phenylacetylglutamine (PAGln) is strongly associated with the prognosis of MI patients.. However, whether PAGln is involved in the regulation of AF after MI is currently unknown.. Therefore, the present study aimed to explore the effect of PAGln on the susceptibility to AF after MI.

    Concentration Assay:

    Article Title: The gut microbial metabolite phenylacetylglutamine increases susceptibility to atrial fibrillation after myocardial infarction through ferroptosis and NLRP3 inflammasome.
    Article Snippet: Myocardial infarction (MI) is an important risk factor for the development of atrial fibrillation (AF), and the gut microbial metabolite phenylacetylglutamine (PAGln) is strongly associated with the prognosis of MI patients.. However, whether PAGln is involved in the regulation of AF after MI is currently unknown.. Therefore, the present study aimed to explore the effect of PAGln on the susceptibility to AF after MI.

    Staining:

    Article Title: Hawthorn leaf flavonoids alleviate the deterioration of atherosclerosis by inhibiting SCAP-SREBP2-LDLR pathway through sPLA2-ⅡA signaling in macrophages in mice.
    Article Snippet: Ethnopharmacological relevance: Hawthorn leaves are a combination of the dried leaves of the Rosaceae plants, i. e., Crataegus pinnatifida Bge. or Crataegus pinnatifida Bge. var. major N. E.. Br., is primarily cultivated in East Asia, North America, and Europe. hawthorn leaf flavonoids (HLF) are the main part of extraction.. The HLF have demonstrated potential in preventing hypertension, inflammation, hyperlipidemia, and atherosclerosis.



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