Virus:Article Title: Pyruvate metabolism enzyme DLAT promotes tumorigenesis by suppressing leucine catabolism.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Rosetta WEIDI Cat# EC1010 TOP10 WEIDI Cat# DL1010 Stbl3 WEIDI Cat# DL1080 Lenti-virus This paper N/A AAV2/9-CBG-DIO-miR30 shNC Heyuan HYKY-240813020 AAV2/9-CBG-DIO-miR30 shRNA (DLAT) Heyuan HYKY-240813020 Biological samples Human Liver Tissue Sample Shanghai Jiao Tong University Affiliated Sixth People’s Hospital N/A Chemicals, peptides, and recombinant proteins Recombinant Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1) Abmart Cat# RKRD68955S DEN Sigma-Aldrich Cat# N0258 b-Methylcrotonyl coenzyme A lithium Sigma-Aldrich Cat# M3013 b-hydroxy-b-mathylglutaryl-CoA Sigma-Aldrich Cat# H6132 L-Valine MedChemExpress Cat# HY-N0717 L-Leucine MedChemExpress Cat# HY-N0486 L-Aspartic acid MedChemExpress Cat# HY-N0666 DMEM basic (Amino acid Free) Yuchun Cat# YC-2082 L-Leucine (13C6; 99%) Cambridge Isotope Laboratories Cat# CLM-2262-H-PK Sodium Pyruvate (13C3; 99%) Cambridge Isotope Laboratories Cat# CLM-2440-PK L-Glutamine (13C5, 99%) Cambridge Isotope Laboratories Cat# CLM-1822-H-MG-PK Acetyl CoA (98%) Yuanye Cat# S23156 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat# L3000015 ECL detection reagent Mine Cat# WB0500 Trypsin Thermo Fisher Scientific Cat# 25200 Fetal Bovine Serum Thermo Fisher Scientific Cat# 16000-044 Protein A/G Magnetic Beads Thermo Fisher Scientific Cat# 88803 Anti-Flag Magnetic Beads Thermo Fisher Scientific Cat# A36797 Fetal Bovine Serum (Dialyzed) Thermo Fisher Scientific Cat# 30067334 PageRuler protein marker Thermo Fisher Scientific Cat# #26616/26619 Matrigel Corning Cat# 356234 DAPI Beyotime Cat# C1002 Deacetylase Inhibitor Cocktail, 100X Beyotime Cat# P1112 Protease Inhibitor Cocktail (100X in DMSO, EDTA Free) Mine Cat# MPI010 Critical commercial assays BCA assay kit Thermo Fisher Scientific Cat# 23225 BeyoClick EdU Cell Proliferation Kit with Alexa Fluor 555 Beyotime Cat# S0511S CCK8 Assay Kit Agilent Cat# 103792-100 Amino Acid Uptake Assay Kit DOJINDO Cat# UP04 Acetlytransferase Activity Kit Enzo Cat# ELS-ADI-907-026 Deposited data Raw and analyzed data Lu et al., 202262 GEO: GSE149614 Raw and analyzed data Kim et al., 201463 GEO: GSE39791 Raw and analyzed data Woo et al., 201764 GEO: GSE87630 Raw and analyzed data Roessler et al., 201065 GEO: GSE14520 Raw and analyzed data Wang et al., 201766 GEO: GSE84402 (Continued on next page) Cell Metabolism 37, 1–19.e1–e9, June 3, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Raw and analyzed data Whitby et al.19 https://doi.org/10.3390/cancers15215182 Acetylated proteomics data related to Figure 5 This paper Mendeley Data (https://doi.org/10.17632/ dsv57bhf65.1) Metabolomics raw data related to Figure 3A This paper Mendeley Data (https://doi.org/10.17632/ 69yx4r728s.1) RNA-seq of Homo sapiens strain: HepG2 cell line (sgDLAT vs sgNC) Raw sequence reads, related to Figure 4.
shRNA:Article Title: Pyruvate metabolism enzyme DLAT promotes tumorigenesis by suppressing leucine catabolism.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Rosetta WEIDI Cat# EC1010 TOP10 WEIDI Cat# DL1010 Stbl3 WEIDI Cat# DL1080 Lenti-virus This paper N/A AAV2/9-CBG-DIO-miR30 shNC Heyuan HYKY-240813020 AAV2/9-CBG-DIO-miR30 shRNA (DLAT) Heyuan HYKY-240813020 Biological samples Human Liver Tissue Sample Shanghai Jiao Tong University Affiliated Sixth People’s Hospital N/A Chemicals, peptides, and recombinant proteins Recombinant Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1) Abmart Cat# RKRD68955S DEN Sigma-Aldrich Cat# N0258 b-Methylcrotonyl coenzyme A lithium Sigma-Aldrich Cat# M3013 b-hydroxy-b-mathylglutaryl-CoA Sigma-Aldrich Cat# H6132 L-Valine MedChemExpress Cat# HY-N0717 L-Leucine MedChemExpress Cat# HY-N0486 L-Aspartic acid MedChemExpress Cat# HY-N0666 DMEM basic (Amino acid Free) Yuchun Cat# YC-2082 L-Leucine (13C6; 99%) Cambridge Isotope Laboratories Cat# CLM-2262-H-PK Sodium Pyruvate (13C3; 99%) Cambridge Isotope Laboratories Cat# CLM-2440-PK L-Glutamine (13C5, 99%) Cambridge Isotope Laboratories Cat# CLM-1822-H-MG-PK Acetyl CoA (98%) Yuanye Cat# S23156 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat# L3000015 ECL detection reagent Mine Cat# WB0500 Trypsin Thermo Fisher Scientific Cat# 25200 Fetal Bovine Serum Thermo Fisher Scientific Cat# 16000-044 Protein A/G Magnetic Beads Thermo Fisher Scientific Cat# 88803 Anti-Flag Magnetic Beads Thermo Fisher Scientific Cat# A36797 Fetal Bovine Serum (Dialyzed) Thermo Fisher Scientific Cat# 30067334 PageRuler protein marker Thermo Fisher Scientific Cat# #26616/26619 Matrigel Corning Cat# 356234 DAPI Beyotime Cat# C1002 Deacetylase Inhibitor Cocktail, 100X Beyotime Cat# P1112 Protease Inhibitor Cocktail (100X in DMSO, EDTA Free) Mine Cat# MPI010 Critical commercial assays BCA assay kit Thermo Fisher Scientific Cat# 23225 BeyoClick EdU Cell Proliferation Kit with Alexa Fluor 555 Beyotime Cat# S0511S CCK8 Assay Kit Agilent Cat# 103792-100 Amino Acid Uptake Assay Kit DOJINDO Cat# UP04 Acetlytransferase Activity Kit Enzo Cat# ELS-ADI-907-026 Deposited data Raw and analyzed data Lu et al., 202262 GEO: GSE149614 Raw and analyzed data Kim et al., 201463 GEO: GSE39791 Raw and analyzed data Woo et al., 201764 GEO: GSE87630 Raw and analyzed data Roessler et al., 201065 GEO: GSE14520 Raw and analyzed data Wang et al., 201766 GEO: GSE84402 (Continued on next page) Cell Metabolism 37, 1–19.e1–e9, June 3, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Raw and analyzed data Whitby et al.19 https://doi.org/10.3390/cancers15215182 Acetylated proteomics data related to Figure 5 This paper Mendeley Data (https://doi.org/10.17632/ dsv57bhf65.1) Metabolomics raw data related to Figure 3A This paper Mendeley Data (https://doi.org/10.17632/ 69yx4r728s.1) RNA-seq of Homo sapiens strain: HepG2 cell line (sgDLAT vs sgNC) Raw sequence reads, related to Figure 4.
Recombinant:Article Title: Pyruvate metabolism enzyme DLAT promotes tumorigenesis by suppressing leucine catabolism.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Rosetta WEIDI Cat# EC1010 TOP10 WEIDI Cat# DL1010 Stbl3 WEIDI Cat# DL1080 Lenti-virus This paper N/A AAV2/9-CBG-DIO-miR30 shNC Heyuan HYKY-240813020 AAV2/9-CBG-DIO-miR30 shRNA (DLAT) Heyuan HYKY-240813020 Biological samples Human Liver Tissue Sample Shanghai Jiao Tong University Affiliated Sixth People’s Hospital N/A Chemicals, peptides, and recombinant proteins Recombinant Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1) Abmart Cat# RKRD68955S DEN Sigma-Aldrich Cat# N0258 b-Methylcrotonyl coenzyme A lithium Sigma-Aldrich Cat# M3013 b-hydroxy-b-mathylglutaryl-CoA Sigma-Aldrich Cat# H6132 L-Valine MedChemExpress Cat# HY-N0717 L-Leucine MedChemExpress Cat# HY-N0486 L-Aspartic acid MedChemExpress Cat# HY-N0666 DMEM basic (Amino acid Free) Yuchun Cat# YC-2082 L-Leucine (13C6; 99%) Cambridge Isotope Laboratories Cat# CLM-2262-H-PK Sodium Pyruvate (13C3; 99%) Cambridge Isotope Laboratories Cat# CLM-2440-PK L-Glutamine (13C5, 99%) Cambridge Isotope Laboratories Cat# CLM-1822-H-MG-PK Acetyl CoA (98%) Yuanye Cat# S23156 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat# L3000015 ECL detection reagent Mine Cat# WB0500 Trypsin Thermo Fisher Scientific Cat# 25200 Fetal Bovine Serum Thermo Fisher Scientific Cat# 16000-044 Protein A/G Magnetic Beads Thermo Fisher Scientific Cat# 88803 Anti-Flag Magnetic Beads Thermo Fisher Scientific Cat# A36797 Fetal Bovine Serum (Dialyzed) Thermo Fisher Scientific Cat# 30067334 PageRuler protein marker Thermo Fisher Scientific Cat# #26616/26619 Matrigel Corning Cat# 356234 DAPI Beyotime Cat# C1002 Deacetylase Inhibitor Cocktail, 100X Beyotime Cat# P1112 Protease Inhibitor Cocktail (100X in DMSO, EDTA Free) Mine Cat# MPI010 Critical commercial assays BCA assay kit Thermo Fisher Scientific Cat# 23225 BeyoClick EdU Cell Proliferation Kit with Alexa Fluor 555 Beyotime Cat# S0511S CCK8 Assay Kit Agilent Cat# 103792-100 Amino Acid Uptake Assay Kit DOJINDO Cat# UP04 Acetlytransferase Activity Kit Enzo Cat# ELS-ADI-907-026 Deposited data Raw and analyzed data Lu et al., 202262 GEO: GSE149614 Raw and analyzed data Kim et al., 201463 GEO: GSE39791 Raw and analyzed data Woo et al., 201764 GEO: GSE87630 Raw and analyzed data Roessler et al., 201065 GEO: GSE14520 Raw and analyzed data Wang et al., 201766 GEO: GSE84402 (Continued on next page) Cell Metabolism 37, 1–19.e1–e9, June 3, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Raw and analyzed data Whitby et al.19 https://doi.org/10.3390/cancers15215182 Acetylated proteomics data related to Figure 5 This paper Mendeley Data (https://doi.org/10.17632/ dsv57bhf65.1) Metabolomics raw data related to Figure 3A This paper Mendeley Data (https://doi.org/10.17632/ 69yx4r728s.1) RNA-seq of Homo sapiens strain: HepG2 cell line (sgDLAT vs sgNC) Raw sequence reads, related to Figure 4.
Transfection:Article Title: Pyruvate metabolism enzyme DLAT promotes tumorigenesis by suppressing leucine catabolism.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Rosetta WEIDI Cat# EC1010 TOP10 WEIDI Cat# DL1010 Stbl3 WEIDI Cat# DL1080 Lenti-virus This paper N/A AAV2/9-CBG-DIO-miR30 shNC Heyuan HYKY-240813020 AAV2/9-CBG-DIO-miR30 shRNA (DLAT) Heyuan HYKY-240813020 Biological samples Human Liver Tissue Sample Shanghai Jiao Tong University Affiliated Sixth People’s Hospital N/A Chemicals, peptides, and recombinant proteins Recombinant Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1) Abmart Cat# RKRD68955S DEN Sigma-Aldrich Cat# N0258 b-Methylcrotonyl coenzyme A lithium Sigma-Aldrich Cat# M3013 b-hydroxy-b-mathylglutaryl-CoA Sigma-Aldrich Cat# H6132 L-Valine MedChemExpress Cat# HY-N0717 L-Leucine MedChemExpress Cat# HY-N0486 L-Aspartic acid MedChemExpress Cat# HY-N0666 DMEM basic (Amino acid Free) Yuchun Cat# YC-2082 L-Leucine (13C6; 99%) Cambridge Isotope Laboratories Cat# CLM-2262-H-PK Sodium Pyruvate (13C3; 99%) Cambridge Isotope Laboratories Cat# CLM-2440-PK L-Glutamine (13C5, 99%) Cambridge Isotope Laboratories Cat# CLM-1822-H-MG-PK Acetyl CoA (98%) Yuanye Cat# S23156 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat# L3000015 ECL detection reagent Mine Cat# WB0500 Trypsin Thermo Fisher Scientific Cat# 25200 Fetal Bovine Serum Thermo Fisher Scientific Cat# 16000-044 Protein A/G Magnetic Beads Thermo Fisher Scientific Cat# 88803 Anti-Flag Magnetic Beads Thermo Fisher Scientific Cat# A36797 Fetal Bovine Serum (Dialyzed) Thermo Fisher Scientific Cat# 30067334 PageRuler protein marker Thermo Fisher Scientific Cat# #26616/26619 Matrigel Corning Cat# 356234 DAPI Beyotime Cat# C1002 Deacetylase Inhibitor Cocktail, 100X Beyotime Cat# P1112 Protease Inhibitor Cocktail (100X in DMSO, EDTA Free) Mine Cat# MPI010 Critical commercial assays BCA assay kit Thermo Fisher Scientific Cat# 23225 BeyoClick EdU Cell Proliferation Kit with Alexa Fluor 555 Beyotime Cat# S0511S CCK8 Assay Kit Agilent Cat# 103792-100 Amino Acid Uptake Assay Kit DOJINDO Cat# UP04 Acetlytransferase Activity Kit Enzo Cat# ELS-ADI-907-026 Deposited data Raw and analyzed data Lu et al., 202262 GEO: GSE149614 Raw and analyzed data Kim et al., 201463 GEO: GSE39791 Raw and analyzed data Woo et al., 201764 GEO: GSE87630 Raw and analyzed data Roessler et al., 201065 GEO: GSE14520 Raw and analyzed data Wang et al., 201766 GEO: GSE84402 (Continued on next page) Cell Metabolism 37, 1–19.e1–e9, June 3, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Raw and analyzed data Whitby et al.19 https://doi.org/10.3390/cancers15215182 Acetylated proteomics data related to Figure 5 This paper Mendeley Data (https://doi.org/10.17632/ dsv57bhf65.1) Metabolomics raw data related to Figure 3A This paper Mendeley Data (https://doi.org/10.17632/ 69yx4r728s.1) RNA-seq of Homo sapiens strain: HepG2 cell line (sgDLAT vs sgNC) Raw sequence reads, related to Figure 4.
Magnetic Beads:Article Title: Pyruvate metabolism enzyme DLAT promotes tumorigenesis by suppressing leucine catabolism.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Rosetta WEIDI Cat# EC1010 TOP10 WEIDI Cat# DL1010 Stbl3 WEIDI Cat# DL1080 Lenti-virus This paper N/A AAV2/9-CBG-DIO-miR30 shNC Heyuan HYKY-240813020 AAV2/9-CBG-DIO-miR30 shRNA (DLAT) Heyuan HYKY-240813020 Biological samples Human Liver Tissue Sample Shanghai Jiao Tong University Affiliated Sixth People’s Hospital N/A Chemicals, peptides, and recombinant proteins Recombinant Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1) Abmart Cat# RKRD68955S DEN Sigma-Aldrich Cat# N0258 b-Methylcrotonyl coenzyme A lithium Sigma-Aldrich Cat# M3013 b-hydroxy-b-mathylglutaryl-CoA Sigma-Aldrich Cat# H6132 L-Valine MedChemExpress Cat# HY-N0717 L-Leucine MedChemExpress Cat# HY-N0486 L-Aspartic acid MedChemExpress Cat# HY-N0666 DMEM basic (Amino acid Free) Yuchun Cat# YC-2082 L-Leucine (13C6; 99%) Cambridge Isotope Laboratories Cat# CLM-2262-H-PK Sodium Pyruvate (13C3; 99%) Cambridge Isotope Laboratories Cat# CLM-2440-PK L-Glutamine (13C5, 99%) Cambridge Isotope Laboratories Cat# CLM-1822-H-MG-PK Acetyl CoA (98%) Yuanye Cat# S23156 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat# L3000015 ECL detection reagent Mine Cat# WB0500 Trypsin Thermo Fisher Scientific Cat# 25200 Fetal Bovine Serum Thermo Fisher Scientific Cat# 16000-044 Protein A/G Magnetic Beads Thermo Fisher Scientific Cat# 88803 Anti-Flag Magnetic Beads Thermo Fisher Scientific Cat# A36797 Fetal Bovine Serum (Dialyzed) Thermo Fisher Scientific Cat# 30067334 PageRuler protein marker Thermo Fisher Scientific Cat# #26616/26619 Matrigel Corning Cat# 356234 DAPI Beyotime Cat# C1002 Deacetylase Inhibitor Cocktail, 100X Beyotime Cat# P1112 Protease Inhibitor Cocktail (100X in DMSO, EDTA Free) Mine Cat# MPI010 Critical commercial assays BCA assay kit Thermo Fisher Scientific Cat# 23225 BeyoClick EdU Cell Proliferation Kit with Alexa Fluor 555 Beyotime Cat# S0511S CCK8 Assay Kit Agilent Cat# 103792-100 Amino Acid Uptake Assay Kit DOJINDO Cat# UP04 Acetlytransferase Activity Kit Enzo Cat# ELS-ADI-907-026 Deposited data Raw and analyzed data Lu et al., 202262 GEO: GSE149614 Raw and analyzed data Kim et al., 201463 GEO: GSE39791 Raw and analyzed data Woo et al., 201764 GEO: GSE87630 Raw and analyzed data Roessler et al., 201065 GEO: GSE14520 Raw and analyzed data Wang et al., 201766 GEO: GSE84402 (Continued on next page) Cell Metabolism 37, 1–19.e1–e9, June 3, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Raw and analyzed data Whitby et al.19 https://doi.org/10.3390/cancers15215182 Acetylated proteomics data related to Figure 5 This paper Mendeley Data (https://doi.org/10.17632/ dsv57bhf65.1) Metabolomics raw data related to Figure 3A This paper Mendeley Data (https://doi.org/10.17632/ 69yx4r728s.1) RNA-seq of Homo sapiens strain: HepG2 cell line (sgDLAT vs sgNC) Raw sequence reads, related to Figure 4.
Marker:Article Title: Pyruvate metabolism enzyme DLAT promotes tumorigenesis by suppressing leucine catabolism.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Rosetta WEIDI Cat# EC1010 TOP10 WEIDI Cat# DL1010 Stbl3 WEIDI Cat# DL1080 Lenti-virus This paper N/A AAV2/9-CBG-DIO-miR30 shNC Heyuan HYKY-240813020 AAV2/9-CBG-DIO-miR30 shRNA (DLAT) Heyuan HYKY-240813020 Biological samples Human Liver Tissue Sample Shanghai Jiao Tong University Affiliated Sixth People’s Hospital N/A Chemicals, peptides, and recombinant proteins Recombinant Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1) Abmart Cat# RKRD68955S DEN Sigma-Aldrich Cat# N0258 b-Methylcrotonyl coenzyme A lithium Sigma-Aldrich Cat# M3013 b-hydroxy-b-mathylglutaryl-CoA Sigma-Aldrich Cat# H6132 L-Valine MedChemExpress Cat# HY-N0717 L-Leucine MedChemExpress Cat# HY-N0486 L-Aspartic acid MedChemExpress Cat# HY-N0666 DMEM basic (Amino acid Free) Yuchun Cat# YC-2082 L-Leucine (13C6; 99%) Cambridge Isotope Laboratories Cat# CLM-2262-H-PK Sodium Pyruvate (13C3; 99%) Cambridge Isotope Laboratories Cat# CLM-2440-PK L-Glutamine (13C5, 99%) Cambridge Isotope Laboratories Cat# CLM-1822-H-MG-PK Acetyl CoA (98%) Yuanye Cat# S23156 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat# L3000015 ECL detection reagent Mine Cat# WB0500 Trypsin Thermo Fisher Scientific Cat# 25200 Fetal Bovine Serum Thermo Fisher Scientific Cat# 16000-044 Protein A/G Magnetic Beads Thermo Fisher Scientific Cat# 88803 Anti-Flag Magnetic Beads Thermo Fisher Scientific Cat# A36797 Fetal Bovine Serum (Dialyzed) Thermo Fisher Scientific Cat# 30067334 PageRuler protein marker Thermo Fisher Scientific Cat# #26616/26619 Matrigel Corning Cat# 356234 DAPI Beyotime Cat# C1002 Deacetylase Inhibitor Cocktail, 100X Beyotime Cat# P1112 Protease Inhibitor Cocktail (100X in DMSO, EDTA Free) Mine Cat# MPI010 Critical commercial assays BCA assay kit Thermo Fisher Scientific Cat# 23225 BeyoClick EdU Cell Proliferation Kit with Alexa Fluor 555 Beyotime Cat# S0511S CCK8 Assay Kit Agilent Cat# 103792-100 Amino Acid Uptake Assay Kit DOJINDO Cat# UP04 Acetlytransferase Activity Kit Enzo Cat# ELS-ADI-907-026 Deposited data Raw and analyzed data Lu et al., 202262 GEO: GSE149614 Raw and analyzed data Kim et al., 201463 GEO: GSE39791 Raw and analyzed data Woo et al., 201764 GEO: GSE87630 Raw and analyzed data Roessler et al., 201065 GEO: GSE14520 Raw and analyzed data Wang et al., 201766 GEO: GSE84402 (Continued on next page) Cell Metabolism 37, 1–19.e1–e9, June 3, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Raw and analyzed data Whitby et al.19 https://doi.org/10.3390/cancers15215182 Acetylated proteomics data related to Figure 5 This paper Mendeley Data (https://doi.org/10.17632/ dsv57bhf65.1) Metabolomics raw data related to Figure 3A This paper Mendeley Data (https://doi.org/10.17632/ 69yx4r728s.1) RNA-seq of Homo sapiens strain: HepG2 cell line (sgDLAT vs sgNC) Raw sequence reads, related to Figure 4.
Histone Deacetylase Assay:Article Title: Pyruvate metabolism enzyme DLAT promotes tumorigenesis by suppressing leucine catabolism.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Rosetta WEIDI Cat# EC1010 TOP10 WEIDI Cat# DL1010 Stbl3 WEIDI Cat# DL1080 Lenti-virus This paper N/A AAV2/9-CBG-DIO-miR30 shNC Heyuan HYKY-240813020 AAV2/9-CBG-DIO-miR30 shRNA (DLAT) Heyuan HYKY-240813020 Biological samples Human Liver Tissue Sample Shanghai Jiao Tong University Affiliated Sixth People’s Hospital N/A Chemicals, peptides, and recombinant proteins Recombinant Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1) Abmart Cat# RKRD68955S DEN Sigma-Aldrich Cat# N0258 b-Methylcrotonyl coenzyme A lithium Sigma-Aldrich Cat# M3013 b-hydroxy-b-mathylglutaryl-CoA Sigma-Aldrich Cat# H6132 L-Valine MedChemExpress Cat# HY-N0717 L-Leucine MedChemExpress Cat# HY-N0486 L-Aspartic acid MedChemExpress Cat# HY-N0666 DMEM basic (Amino acid Free) Yuchun Cat# YC-2082 L-Leucine (13C6; 99%) Cambridge Isotope Laboratories Cat# CLM-2262-H-PK Sodium Pyruvate (13C3; 99%) Cambridge Isotope Laboratories Cat# CLM-2440-PK L-Glutamine (13C5, 99%) Cambridge Isotope Laboratories Cat# CLM-1822-H-MG-PK Acetyl CoA (98%) Yuanye Cat# S23156 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat# L3000015 ECL detection reagent Mine Cat# WB0500 Trypsin Thermo Fisher Scientific Cat# 25200 Fetal Bovine Serum Thermo Fisher Scientific Cat# 16000-044 Protein A/G Magnetic Beads Thermo Fisher Scientific Cat# 88803 Anti-Flag Magnetic Beads Thermo Fisher Scientific Cat# A36797 Fetal Bovine Serum (Dialyzed) Thermo Fisher Scientific Cat# 30067334 PageRuler protein marker Thermo Fisher Scientific Cat# #26616/26619 Matrigel Corning Cat# 356234 DAPI Beyotime Cat# C1002 Deacetylase Inhibitor Cocktail, 100X Beyotime Cat# P1112 Protease Inhibitor Cocktail (100X in DMSO, EDTA Free) Mine Cat# MPI010 Critical commercial assays BCA assay kit Thermo Fisher Scientific Cat# 23225 BeyoClick EdU Cell Proliferation Kit with Alexa Fluor 555 Beyotime Cat# S0511S CCK8 Assay Kit Agilent Cat# 103792-100 Amino Acid Uptake Assay Kit DOJINDO Cat# UP04 Acetlytransferase Activity Kit Enzo Cat# ELS-ADI-907-026 Deposited data Raw and analyzed data Lu et al., 202262 GEO: GSE149614 Raw and analyzed data Kim et al., 201463 GEO: GSE39791 Raw and analyzed data Woo et al., 201764 GEO: GSE87630 Raw and analyzed data Roessler et al., 201065 GEO: GSE14520 Raw and analyzed data Wang et al., 201766 GEO: GSE84402 (Continued on next page) Cell Metabolism 37, 1–19.e1–e9, June 3, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Raw and analyzed data Whitby et al.19 https://doi.org/10.3390/cancers15215182 Acetylated proteomics data related to Figure 5 This paper Mendeley Data (https://doi.org/10.17632/ dsv57bhf65.1) Metabolomics raw data related to Figure 3A This paper Mendeley Data (https://doi.org/10.17632/ 69yx4r728s.1) RNA-seq of Homo sapiens strain: HepG2 cell line (sgDLAT vs sgNC) Raw sequence reads, related to Figure 4.
Protease Inhibitor:Article Title: Pyruvate metabolism enzyme DLAT promotes tumorigenesis by suppressing leucine catabolism.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Rosetta WEIDI Cat# EC1010 TOP10 WEIDI Cat# DL1010 Stbl3 WEIDI Cat# DL1080 Lenti-virus This paper N/A AAV2/9-CBG-DIO-miR30 shNC Heyuan HYKY-240813020 AAV2/9-CBG-DIO-miR30 shRNA (DLAT) Heyuan HYKY-240813020 Biological samples Human Liver Tissue Sample Shanghai Jiao Tong University Affiliated Sixth People’s Hospital N/A Chemicals, peptides, and recombinant proteins Recombinant Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1) Abmart Cat# RKRD68955S DEN Sigma-Aldrich Cat# N0258 b-Methylcrotonyl coenzyme A lithium Sigma-Aldrich Cat# M3013 b-hydroxy-b-mathylglutaryl-CoA Sigma-Aldrich Cat# H6132 L-Valine MedChemExpress Cat# HY-N0717 L-Leucine MedChemExpress Cat# HY-N0486 L-Aspartic acid MedChemExpress Cat# HY-N0666 DMEM basic (Amino acid Free) Yuchun Cat# YC-2082 L-Leucine (13C6; 99%) Cambridge Isotope Laboratories Cat# CLM-2262-H-PK Sodium Pyruvate (13C3; 99%) Cambridge Isotope Laboratories Cat# CLM-2440-PK L-Glutamine (13C5, 99%) Cambridge Isotope Laboratories Cat# CLM-1822-H-MG-PK Acetyl CoA (98%) Yuanye Cat# S23156 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat# L3000015 ECL detection reagent Mine Cat# WB0500 Trypsin Thermo Fisher Scientific Cat# 25200 Fetal Bovine Serum Thermo Fisher Scientific Cat# 16000-044 Protein A/G Magnetic Beads Thermo Fisher Scientific Cat# 88803 Anti-Flag Magnetic Beads Thermo Fisher Scientific Cat# A36797 Fetal Bovine Serum (Dialyzed) Thermo Fisher Scientific Cat# 30067334 PageRuler protein marker Thermo Fisher Scientific Cat# #26616/26619 Matrigel Corning Cat# 356234 DAPI Beyotime Cat# C1002 Deacetylase Inhibitor Cocktail, 100X Beyotime Cat# P1112 Protease Inhibitor Cocktail (100X in DMSO, EDTA Free) Mine Cat# MPI010 Critical commercial assays BCA assay kit Thermo Fisher Scientific Cat# 23225 BeyoClick EdU Cell Proliferation Kit with Alexa Fluor 555 Beyotime Cat# S0511S CCK8 Assay Kit Agilent Cat# 103792-100 Amino Acid Uptake Assay Kit DOJINDO Cat# UP04 Acetlytransferase Activity Kit Enzo Cat# ELS-ADI-907-026 Deposited data Raw and analyzed data Lu et al., 202262 GEO: GSE149614 Raw and analyzed data Kim et al., 201463 GEO: GSE39791 Raw and analyzed data Woo et al., 201764 GEO: GSE87630 Raw and analyzed data Roessler et al., 201065 GEO: GSE14520 Raw and analyzed data Wang et al., 201766 GEO: GSE84402 (Continued on next page) Cell Metabolism 37, 1–19.e1–e9, June 3, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Raw and analyzed data Whitby et al.19 https://doi.org/10.3390/cancers15215182 Acetylated proteomics data related to Figure 5 This paper Mendeley Data (https://doi.org/10.17632/ dsv57bhf65.1) Metabolomics raw data related to Figure 3A This paper Mendeley Data (https://doi.org/10.17632/ 69yx4r728s.1) RNA-seq of Homo sapiens strain: HepG2 cell line (sgDLAT vs sgNC) Raw sequence reads, related to Figure 4.
BIA-KA:Article Title: Pyruvate metabolism enzyme DLAT promotes tumorigenesis by suppressing leucine catabolism.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Rosetta WEIDI Cat# EC1010 TOP10 WEIDI Cat# DL1010 Stbl3 WEIDI Cat# DL1080 Lenti-virus This paper N/A AAV2/9-CBG-DIO-miR30 shNC Heyuan HYKY-240813020 AAV2/9-CBG-DIO-miR30 shRNA (DLAT) Heyuan HYKY-240813020 Biological samples Human Liver Tissue Sample Shanghai Jiao Tong University Affiliated Sixth People’s Hospital N/A Chemicals, peptides, and recombinant proteins Recombinant Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1) Abmart Cat# RKRD68955S DEN Sigma-Aldrich Cat# N0258 b-Methylcrotonyl coenzyme A lithium Sigma-Aldrich Cat# M3013 b-hydroxy-b-mathylglutaryl-CoA Sigma-Aldrich Cat# H6132 L-Valine MedChemExpress Cat# HY-N0717 L-Leucine MedChemExpress Cat# HY-N0486 L-Aspartic acid MedChemExpress Cat# HY-N0666 DMEM basic (Amino acid Free) Yuchun Cat# YC-2082 L-Leucine (13C6; 99%) Cambridge Isotope Laboratories Cat# CLM-2262-H-PK Sodium Pyruvate (13C3; 99%) Cambridge Isotope Laboratories Cat# CLM-2440-PK L-Glutamine (13C5, 99%) Cambridge Isotope Laboratories Cat# CLM-1822-H-MG-PK Acetyl CoA (98%) Yuanye Cat# S23156 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat# L3000015 ECL detection reagent Mine Cat# WB0500 Trypsin Thermo Fisher Scientific Cat# 25200 Fetal Bovine Serum Thermo Fisher Scientific Cat# 16000-044 Protein A/G Magnetic Beads Thermo Fisher Scientific Cat# 88803 Anti-Flag Magnetic Beads Thermo Fisher Scientific Cat# A36797 Fetal Bovine Serum (Dialyzed) Thermo Fisher Scientific Cat# 30067334 PageRuler protein marker Thermo Fisher Scientific Cat# #26616/26619 Matrigel Corning Cat# 356234 DAPI Beyotime Cat# C1002 Deacetylase Inhibitor Cocktail, 100X Beyotime Cat# P1112 Protease Inhibitor Cocktail (100X in DMSO, EDTA Free) Mine Cat# MPI010 Critical commercial assays BCA assay kit Thermo Fisher Scientific Cat# 23225 BeyoClick EdU Cell Proliferation Kit with Alexa Fluor 555 Beyotime Cat# S0511S CCK8 Assay Kit Agilent Cat# 103792-100 Amino Acid Uptake Assay Kit DOJINDO Cat# UP04 Acetlytransferase Activity Kit Enzo Cat# ELS-ADI-907-026 Deposited data Raw and analyzed data Lu et al., 202262 GEO: GSE149614 Raw and analyzed data Kim et al., 201463 GEO: GSE39791 Raw and analyzed data Woo et al., 201764 GEO: GSE87630 Raw and analyzed data Roessler et al., 201065 GEO: GSE14520 Raw and analyzed data Wang et al., 201766 GEO: GSE84402 (Continued on next page) Cell Metabolism 37, 1–19.e1–e9, June 3, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Raw and analyzed data Whitby et al.19 https://doi.org/10.3390/cancers15215182 Acetylated proteomics data related to Figure 5 This paper Mendeley Data (https://doi.org/10.17632/ dsv57bhf65.1) Metabolomics raw data related to Figure 3A This paper Mendeley Data (https://doi.org/10.17632/ 69yx4r728s.1) RNA-seq of Homo sapiens strain: HepG2 cell line (sgDLAT vs sgNC) Raw sequence reads, related to Figure 4.
CCK-8 Assay:Article Title: Pyruvate metabolism enzyme DLAT promotes tumorigenesis by suppressing leucine catabolism.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Rosetta WEIDI Cat# EC1010 TOP10 WEIDI Cat# DL1010 Stbl3 WEIDI Cat# DL1080 Lenti-virus This paper N/A AAV2/9-CBG-DIO-miR30 shNC Heyuan HYKY-240813020 AAV2/9-CBG-DIO-miR30 shRNA (DLAT) Heyuan HYKY-240813020 Biological samples Human Liver Tissue Sample Shanghai Jiao Tong University Affiliated Sixth People’s Hospital N/A Chemicals, peptides, and recombinant proteins Recombinant Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1) Abmart Cat# RKRD68955S DEN Sigma-Aldrich Cat# N0258 b-Methylcrotonyl coenzyme A lithium Sigma-Aldrich Cat# M3013 b-hydroxy-b-mathylglutaryl-CoA Sigma-Aldrich Cat# H6132 L-Valine MedChemExpress Cat# HY-N0717 L-Leucine MedChemExpress Cat# HY-N0486 L-Aspartic acid MedChemExpress Cat# HY-N0666 DMEM basic (Amino acid Free) Yuchun Cat# YC-2082 L-Leucine (13C6; 99%) Cambridge Isotope Laboratories Cat# CLM-2262-H-PK Sodium Pyruvate (13C3; 99%) Cambridge Isotope Laboratories Cat# CLM-2440-PK L-Glutamine (13C5, 99%) Cambridge Isotope Laboratories Cat# CLM-1822-H-MG-PK Acetyl CoA (98%) Yuanye Cat# S23156 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat# L3000015 ECL detection reagent Mine Cat# WB0500 Trypsin Thermo Fisher Scientific Cat# 25200 Fetal Bovine Serum Thermo Fisher Scientific Cat# 16000-044 Protein A/G Magnetic Beads Thermo Fisher Scientific Cat# 88803 Anti-Flag Magnetic Beads Thermo Fisher Scientific Cat# A36797 Fetal Bovine Serum (Dialyzed) Thermo Fisher Scientific Cat# 30067334 PageRuler protein marker Thermo Fisher Scientific Cat# #26616/26619 Matrigel Corning Cat# 356234 DAPI Beyotime Cat# C1002 Deacetylase Inhibitor Cocktail, 100X Beyotime Cat# P1112 Protease Inhibitor Cocktail (100X in DMSO, EDTA Free) Mine Cat# MPI010 Critical commercial assays BCA assay kit Thermo Fisher Scientific Cat# 23225 BeyoClick EdU Cell Proliferation Kit with Alexa Fluor 555 Beyotime Cat# S0511S CCK8 Assay Kit Agilent Cat# 103792-100 Amino Acid Uptake Assay Kit DOJINDO Cat# UP04 Acetlytransferase Activity Kit Enzo Cat# ELS-ADI-907-026 Deposited data Raw and analyzed data Lu et al., 202262 GEO: GSE149614 Raw and analyzed data Kim et al., 201463 GEO: GSE39791 Raw and analyzed data Woo et al., 201764 GEO: GSE87630 Raw and analyzed data Roessler et al., 201065 GEO: GSE14520 Raw and analyzed data Wang et al., 201766 GEO: GSE84402 (Continued on next page) Cell Metabolism 37, 1–19.e1–e9, June 3, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Raw and analyzed data Whitby et al.19 https://doi.org/10.3390/cancers15215182 Acetylated proteomics data related to Figure 5 This paper Mendeley Data (https://doi.org/10.17632/ dsv57bhf65.1) Metabolomics raw data related to Figure 3A This paper Mendeley Data (https://doi.org/10.17632/ 69yx4r728s.1) RNA-seq of Homo sapiens strain: HepG2 cell line (sgDLAT vs sgNC) Raw sequence reads, related to Figure 4.
Activity Assay:Article Title: Pyruvate metabolism enzyme DLAT promotes tumorigenesis by suppressing leucine catabolism.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Rosetta WEIDI Cat# EC1010 TOP10 WEIDI Cat# DL1010 Stbl3 WEIDI Cat# DL1080 Lenti-virus This paper N/A AAV2/9-CBG-DIO-miR30 shNC Heyuan HYKY-240813020 AAV2/9-CBG-DIO-miR30 shRNA (DLAT) Heyuan HYKY-240813020 Biological samples Human Liver Tissue Sample Shanghai Jiao Tong University Affiliated Sixth People’s Hospital N/A Chemicals, peptides, and recombinant proteins Recombinant Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1) Abmart Cat# RKRD68955S DEN Sigma-Aldrich Cat# N0258 b-Methylcrotonyl coenzyme A lithium Sigma-Aldrich Cat# M3013 b-hydroxy-b-mathylglutaryl-CoA Sigma-Aldrich Cat# H6132 L-Valine MedChemExpress Cat# HY-N0717 L-Leucine MedChemExpress Cat# HY-N0486 L-Aspartic acid MedChemExpress Cat# HY-N0666 DMEM basic (Amino acid Free) Yuchun Cat# YC-2082 L-Leucine (13C6; 99%) Cambridge Isotope Laboratories Cat# CLM-2262-H-PK Sodium Pyruvate (13C3; 99%) Cambridge Isotope Laboratories Cat# CLM-2440-PK L-Glutamine (13C5, 99%) Cambridge Isotope Laboratories Cat# CLM-1822-H-MG-PK Acetyl CoA (98%) Yuanye Cat# S23156 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat# L3000015 ECL detection reagent Mine Cat# WB0500 Trypsin Thermo Fisher Scientific Cat# 25200 Fetal Bovine Serum Thermo Fisher Scientific Cat# 16000-044 Protein A/G Magnetic Beads Thermo Fisher Scientific Cat# 88803 Anti-Flag Magnetic Beads Thermo Fisher Scientific Cat# A36797 Fetal Bovine Serum (Dialyzed) Thermo Fisher Scientific Cat# 30067334 PageRuler protein marker Thermo Fisher Scientific Cat# #26616/26619 Matrigel Corning Cat# 356234 DAPI Beyotime Cat# C1002 Deacetylase Inhibitor Cocktail, 100X Beyotime Cat# P1112 Protease Inhibitor Cocktail (100X in DMSO, EDTA Free) Mine Cat# MPI010 Critical commercial assays BCA assay kit Thermo Fisher Scientific Cat# 23225 BeyoClick EdU Cell Proliferation Kit with Alexa Fluor 555 Beyotime Cat# S0511S CCK8 Assay Kit Agilent Cat# 103792-100 Amino Acid Uptake Assay Kit DOJINDO Cat# UP04 Acetlytransferase Activity Kit Enzo Cat# ELS-ADI-907-026 Deposited data Raw and analyzed data Lu et al., 202262 GEO: GSE149614 Raw and analyzed data Kim et al., 201463 GEO: GSE39791 Raw and analyzed data Woo et al., 201764 GEO: GSE87630 Raw and analyzed data Roessler et al., 201065 GEO: GSE14520 Raw and analyzed data Wang et al., 201766 GEO: GSE84402 (Continued on next page) Cell Metabolism 37, 1–19.e1–e9, June 3, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Raw and analyzed data Whitby et al.19 https://doi.org/10.3390/cancers15215182 Acetylated proteomics data related to Figure 5 This paper Mendeley Data (https://doi.org/10.17632/ dsv57bhf65.1) Metabolomics raw data related to Figure 3A This paper Mendeley Data (https://doi.org/10.17632/ 69yx4r728s.1) RNA-seq of Homo sapiens strain: HepG2 cell line (sgDLAT vs sgNC) Raw sequence reads, related to Figure 4.
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